| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.064637 |
| Chromosome: | chromosome 16 |
| Location: | 4989468 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g686250 | (1 of 24) PTHR10641 - MYB-LIKE DNA-BINDING PROTEIN MYB | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCCCGCTAGCTCTGGTAGGCTGGGGGCGTGCGGAGCGGTGCCGCCCAGT |
| Internal bar code: | TTCAGCTCCGGAGGCGGAGAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1905 |
| LEAP-Seq percent confirming: | 95.5556 |
| LEAP-Seq n confirming: | 43 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 45 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATCTGACGGTATCGCGTCA |
| Suggested primer 2: | GTGGATGTGCAGAGGCTCTT |