| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.064641 |
| Chromosome: | chromosome 12 |
| Location: | 6530361 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g537950 | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGGAGGCCACTCTCCGGGGTGTGACGATAGCCTCTTTTTCATGACAGGT |
| Internal bar code: | CAGTTTTGATGCGCACGTAATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3743 |
| LEAP-Seq percent confirming: | 87.8049 |
| LEAP-Seq n confirming: | 72 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 82 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGTCACACCTTGTTTGGTC |
| Suggested primer 2: | GAGGTGTTGGGTTGGGTAGG |