| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.064653 |
| Chromosome: | chromosome 6 |
| Location: | 2943913 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g273400 | TMC2 | putative tRNA (cytosine-5)-methyltransferase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCCAGCCCTCCTGCGCGTCATGCATACAAGGGTACCCACACTCATGCAT |
| Internal bar code: | ATGCCTTGTGGGCATATGGGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1132 |
| LEAP-Seq percent confirming: | 96.6667 |
| LEAP-Seq n confirming: | 29 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGGGCTGCATGGTGTATGT |
| Suggested primer 2: | CTGGGAGGAAAGGCGGATTT |