| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.064656 |
| Chromosome: | chromosome 12 |
| Location: | 3223777 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g499800 | ASA10 | Mitochondrial F1F0 ATP synthase associated protein 10 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTTTGACAGCCTGGTAGACCCAACAGGTATCATCTGACCAGGCCGCCAG |
| Internal bar code: | GACGTGTTTCCTGCAGTTGCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1861 |
| LEAP-Seq percent confirming: | 20.5128 |
| LEAP-Seq n confirming: | 8 |
| LEAP-Seq n nonconfirming: | 31 |
| LEAP-Seq n unique pos: | 39 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACGGCACTAGATACAGGCC |
| Suggested primer 2: | TACCTGAACACGTTCACCGG |