| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.064709 |
| Chromosome: | chromosome 6 |
| Location: | 827410 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g255450 | MAT3,RB | (1 of 1) K04681 - retinoblastoma-like protein 1 (RBL1); Retinoblastoma protein | 3'UTR |
| Cre06.g255500 | (1 of 1) PTHR10992:SF743 - ALPHA/BETA-HYDROLASE DOMAIN-CONTAINING PROTEIN | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTATAAGTTCAAGCCAAGCGTGTACTGCACACGCATGGACCTGCTCCCAA |
| Internal bar code: | GGCGAATTATAACCTTTTGCCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4804 |
| LEAP-Seq percent confirming: | 98.3051 |
| LEAP-Seq n confirming: | 58 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 59 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGGCCGGTTGCAAAGATTT |
| Suggested primer 2: | GAACCAGCCTTCGTAGGGAG |