| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.064731 |
| Chromosome: | chromosome 10 |
| Location: | 6694975 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g466550 | TF2H1 | General transcription factor IIH subunit 1; (1 of 1) IPR005607//IPR027079 - BSD domain // TFIIH subunit Tfb1/p62 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTTGACCTGGGGCTAGGTGCATGCCGTCTGCCCCGATGAGTTGTGACCC |
| Internal bar code: | ATTGTCGGCTGATACTGCGATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2364 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 27 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCCGTGTGATGTCCACTAA |
| Suggested primer 2: | CAGCCTGTCTCTCTTGACCC |