| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.064739 |
| Chromosome: | chromosome 8 |
| Location: | 3803385 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g381250 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGGAGTTTCCTCGCGGTGCCCGGGAGGGATTCGCGCTAGAAGCTGCTAG |
| Internal bar code: | TGAGTCTGCAGGAGCGGTGATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5415 |
| LEAP-Seq percent confirming: | 13.9535 |
| LEAP-Seq n confirming: | 12 |
| LEAP-Seq n nonconfirming: | 74 |
| LEAP-Seq n unique pos: | 86 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGGCTGTAACTGTAAGGCA |
| Suggested primer 2: | GATAGATGGGGGCGGACAAG |