| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.064758 |
| Chromosome: | chromosome 4 |
| Location: | 2076231 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g219500 | DNJ30 | (1 of 1) PTHR22904:SF338 - LD24721P; DnaJ-like protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCAGGGAAAGGTCCCGAGTTCAATCCTCGGTGGGAGCAGACTTTTGTAT |
| Internal bar code: | TGGCCGGAGAAATAAAGGATAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2330 |
| LEAP-Seq percent confirming: | 93.1034 |
| LEAP-Seq n confirming: | 54 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 58 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCATCAGTGTCAGTGTCCG |
| Suggested primer 2: | CCGCAGAAATTTGCCCCAAA |