Insertion junction: CLIP2.064765_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.064765
Chromosome:chromosome 1
Location:4410352
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g030050 PRPL34 Chloroplast ribosomal protein L34; (1 of 1) K02914 - large subunit ribosomal protein L34 (RP-L34, MRPL34, rpmH) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TACTAAGTGCACCATGGGTCTATGACGGCATGACCACCATGACCAGCACT
Internal bar code:CCTCATAATGGCGTGTGTATGG

Confirmation - LEAP-Seq

LEAP-Seq distance:2748
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:6
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:TGCAGTGTGTGTCAGTGTGT
Suggested primer 2:CATGGACTTAATGCGCCGTG