| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.064796 |
| Chromosome: | chromosome 1 |
| Location: | 3180101 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g020223 | FUM2 | (1 of 1) K01679 - fumarate hydratase, class II (E4.2.1.2B, fumC); Fumarate hydratase 2 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTAGGTTGATGCCTGGGTTGATGCATAGCAGGTATGGGCAGCTGGAAAG |
| Internal bar code: | GCGCTGGTAGCGTACATAAAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1398 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 7 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGTCAAACACGCTCGCTTG |
| Suggested primer 2: | TCTTGCACTTGAAACGCACG |