Insertion junction: CLIP2.064800_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.064800
Chromosome:chromosome 15
Location:953206
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre15.g639650 RAP24,OPR92,NCL11 Nuclear Control of chloroplast Like 11 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCACCTCAGTCGTGGTCGACGGGTGTCAAATGACGCGGGCAGGGACTCA
Internal bar code:CTAAGACGGTCCTGCTGAAGGC

Confirmation - LEAP-Seq

LEAP-Seq distance:2096
LEAP-Seq percent confirming:23.6842
LEAP-Seq n confirming:9
LEAP-Seq n nonconfirming:29
LEAP-Seq n unique pos:38

Suggested primers for confirmation by PCR

Suggested primer 1:TGCTACTGTTGAGTGCTGGG
Suggested primer 2:TGGATGCTAGTGGATGGGGA