| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | - |
| Strain: | CLIP2.064803 |
| Chromosome: | chromosome 3 |
| Location: | 8509011 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g800400 | (1 of 2) PF05139 - Erythromycin esterase (Erythro_esteras) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGGTGAGTGAGTGAGTGGCTGCAGCAGAGAAGAACTCACGGTTGAAGAG |
| Internal bar code: | AGTATGGTGGTCTACGACGAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 895 |
| LEAP-Seq percent confirming: | 23.0769 |
| LEAP-Seq n confirming: | 6 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGTCCAGGACAAACACGTC |
| Suggested primer 2: | AACATAAGGCGGGACTTGGG |