Insertion junction: CLIP2.064812_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.064812
Chromosome:chromosome 11
Location:543435
Confidence (%):80
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCACATTTAAACCATCATTTCCTCTTAGCTTGCAACCATAACAAGTGCGC
Internal bar code:CTCGGGATGTCGCGGTGGCGTA

Confirmation - LEAP-Seq

LEAP-Seq distance:190
LEAP-Seq percent confirming:6.66667
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:14
LEAP-Seq n unique pos:15

Suggested primers for confirmation by PCR

Suggested primer 1:CGGATGATGCAGTAGGGGTC
Suggested primer 2:CGGATGATGCAGTAGGGGTC