| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.064820 |
| Chromosome: | chromosome 16 |
| Location: | 296331 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g801865 | (1 of 1) 1.14.11.42 - tRNA(Phe) (7-(3-amino-3-carboxypropyl)wyosine(37)-C(2))-hydroxylase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGTAGTGTCGCGTTAATTACTTCAGCTGCATGTCTCCAGCAAACGCGTG |
| Internal bar code: | CGGGTAATGCTGCAGCCAATCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1182 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 19 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTTTGGCCCAAGGGTCTTT |
| Suggested primer 2: | GCCCCTGTAGACAAGCCATT |