Insertion junction: CLIP2.064827_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.064827
Chromosome:chromosome 16
Location:7426662
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g680902 (1 of 40) 3.4.24.38 - Gametolysin / Lysin 3'UTR
Cre16.g681014 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGATGACATACGTCTCGCCTGCCGACCTTCATACACACGACCATGCTAG
Internal bar code:AGCAGGGGGTTGGCAGAGATAT

Confirmation - LEAP-Seq

LEAP-Seq distance:1834
LEAP-Seq percent confirming:92.8571
LEAP-Seq n confirming:26
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:28

Suggested primers for confirmation by PCR

Suggested primer 1:GTACGATGGGAGCGAGTGAG
Suggested primer 2:GTGGCAAAGAGGGGGCTTAT