Insertion junction: CLIP2.064854_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.064854
Chromosome:chromosome 7
Location:6145927
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g355900 3'UTR
Cre07.g355950 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAGGGTTTCCGCGGACTGGGGACTCCAATCAACGTTTTCGCCAGCGCTGC
Internal bar code:CCGCCGTTTGAGGATTCGAGAA

Confirmation - LEAP-Seq

LEAP-Seq distance:1570
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:15
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:15

Suggested primers for confirmation by PCR

Suggested primer 1:AGCGCACAGTAAGTTCAGCT
Suggested primer 2:CAGGGGCTTCTTAGACTGCC