Insertion junction: CLIP2.064871_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.064871
Chromosome:chromosome 6
Location:7237728
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g298800 ARS73A,ARS73a,LTH5 Arylsulfatase 73a 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGATGTAAGTAGCCACAACAATGTACATAAAGCCACACACACCTTCTCCG
Internal bar code:TTTCTATATGTGACCGCCATCG

Confirmation - LEAP-Seq

LEAP-Seq distance:1452
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:19
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:19

Suggested primers for confirmation by PCR

Suggested primer 1:GAACGGCTCCAGAACCTGAA
Suggested primer 2:CGGCCAAACTGCATTATCGG