| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.064875 |
| Chromosome: | chromosome 6 |
| Location: | 6735301 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g295450 | HPR1 | (1 of 1) 1.1.1.29 - Glycerate dehydrogenase / Hydroxypyruvate reductase; Hydroxyl pyruvate reductase | 3'UTR |
| Cre06.g295500 | (1 of 33) IPR013830 - SGNH hydrolase-type esterase domain | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTAACACAAATACTTCCTCGAGTTACCGAGCACTCAGCAGAATAGCCAAT |
| Internal bar code: | CGCAAGAAACTGAAGAGTCCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2575 |
| LEAP-Seq percent confirming: | 4.16667 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGATACCTGCAAACATGCGG |
| Suggested primer 2: | GATATGGGTATGGGCGTGGG |