| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.064892 |
| Chromosome: | chromosome 17 |
| Location: | 244519 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g697550 | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGTGAGGCGAGGGCGACAGCGATGCAGGTCAGTTACCATTGAACATGAA |
| Internal bar code: | ACTTATTGTGGCAGAGGTTTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 364 |
| LEAP-Seq percent confirming: | 58.3333 |
| LEAP-Seq n confirming: | 28 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 48 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTTGTTCTTCCACAGGCGC |
| Suggested primer 2: | GACAAGGGAGATGGGGTTGG |