| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.064897 |
| Chromosome: | chromosome 3 |
| Location: | 6571774 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g194535 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGCAAGCGGTCAGATGTCCAAACTTCACGCGCCCGCACTGCACCAACGC |
| Internal bar code: | CTTTCAGTTAGGGTTAGTCTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3912 |
| LEAP-Seq percent confirming: | 82.3529 |
| LEAP-Seq n confirming: | 70 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 85 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTCGTACTCTCAGCTGTCG |
| Suggested primer 2: | CTTCGCCTTCGCTAACATGC |