| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.064904 |
| Chromosome: | chromosome 1 |
| Location: | 3659429 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g023773 | (1 of 6) IPR000253//IPR008984 - Forkhead-associated (FHA) domain // SMAD/FHA domain | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGATGGAAGGGGGCTTCGAAGGGCCATGGCCAGGGCATGCCAGGGGCACG |
| Internal bar code: | GCAATTTCTGAGCCCGGTGCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 847 |
| LEAP-Seq percent confirming: | 90.9091 |
| LEAP-Seq n confirming: | 20 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTATCCGCACGTCGCTACTC |
| Suggested primer 2: | AAGACTGATGGGCATTGGGG |