| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.064906 |
| Chromosome: | chromosome 2 |
| Location: | 1440625 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g083550 | (1 of 1) PF01494//PF05834 - FAD binding domain (FAD_binding_3) // Lycopene cyclase protein (Lycopene_cycl) | 3'UTR | |
| Cre02.g083600 | (1 of 1) PTHR23305//PTHR23305:SF7 - GTP-BINDING PROTEIN-RELATED // SUBFAMILY NOT NAMED | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCAATACAAGTAACTGGTGCAATACAAACAGTGCTGTACTTGCTTCTTTA |
| Internal bar code: | ATCTCTGAACTCTGGTCGCACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1999 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 5 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAATGGGGAAGAGCAGGAGC |
| Suggested primer 2: | CTGTGGAGCAGGAGGATGTG |