| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.064918 |
| Chromosome: | chromosome 3 |
| Location: | 291240 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g800283 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGATGAGACGCACGGGAAGAAATGAGATCTCAACATCAGCCTGAGCATG |
| Internal bar code: | GATAGCTATAAGCAGAGCCTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 910 |
| LEAP-Seq percent confirming: | 58.8235 |
| LEAP-Seq n confirming: | 20 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 34 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGAGTCGCTGCTTGTGCTA |
| Suggested primer 2: | CCAAGGCCCGTTATGTTCCT |