| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.064927 |
| Chromosome: | chromosome 8 |
| Location: | 2236888 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g370401 | CYCB1,DIV80 | (1 of 1) K05868 - cyclin B (CCNB); B-type cyclin | 3'UTR |
| Cre08.g370450 | MGE1 | (1 of 1) PTHR21237//PTHR21237:SF23 - GRPE PROTEIN // GRPE PROTEIN HOMOLOG, MITOCHONDRIAL; Mitochondrial GrpE homolog | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGGTTCGCCATGTGGGCAATGCGATACTGCGTTGCCCGCGCCTGCGCGC |
| Internal bar code: | ATGACAAGTAGGGCTCTACCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2391 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 23 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTCCCCGTCTTGCAGAAAG |
| Suggested primer 2: | GTCCAGCTACCTCATCGAGC |