Insertion junction: CLIP2.064931_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.064931
Chromosome:chromosome 17
Location:1878147
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g709600 3'UTR
Cre17.g802046 (1 of 7) PF00415//PF13540 - Regulator of chromosome condensation (RCC1) repeat (RCC1) // Regulator of chromosome condensation (RCC1) repeat (RCC1_2) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTGGCACAGGGGGGGCCGGGACAACGAGGAGAGGCGGAGAGTAGCGGCTG
Internal bar code:TTAGTTGTCGCAGTTGGTGTCA

Confirmation - LEAP-Seq

LEAP-Seq distance:976
LEAP-Seq percent confirming:91.6667
LEAP-Seq n confirming:11
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:CAGGCACAACAACGAAGCTC
Suggested primer 2:ACACTAGTACACGCAACGCA