| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.064932 |
| Chromosome: | chromosome 3 |
| Location: | 6854908 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g197600 | (1 of 1) PTHR12972//PTHR12972:SF0 - DOWNSTREAM NEIGHBOR OF SON // PROTEIN DOWNSTREAM NEIGHBOR OF SON | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCCGGCATCAGCACGGCGGAATAGCAACTGAGTGAACTTGCGCGTGGCA |
| Internal bar code: | ACGTTTCACGTGCAGGTAAACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3955 |
| LEAP-Seq percent confirming: | 65.7534 |
| LEAP-Seq n confirming: | 48 |
| LEAP-Seq n nonconfirming: | 25 |
| LEAP-Seq n unique pos: | 73 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTAGTGCGCGACATTGAAG |
| Suggested primer 2: | GCTGACCGTGTTGTCAAACC |