| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.064947 |
| Chromosome: | chromosome 9 |
| Location: | 432932 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre18.g749497 | (1 of 1) K01945 - phosphoribosylamine--glycine ligase (purD) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGATACACTGTACGCCCCGGGTACGATTGTGTGACGTGCTTCAAACTGT |
| Internal bar code: | ACTGAGTCTCAGACGGACGGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 183 |
| LEAP-Seq percent confirming: | 10.0 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTTGAGCAAGTCCCGTCAC |
| Suggested primer 2: | CAGCGGGACACACCTTTTTG |