| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.064953 |
| Chromosome: | chromosome 3 |
| Location: | 293758 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g144444 | (1 of 1) IPR022416 - Prion/Doppel protein, beta-ribbon domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCGCATTGACGTTGGACATGACGGATGGGTAACCGAATGACGAACACAT |
| Internal bar code: | GCTAGAGGTCACTCAGTCGCTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2899 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 18 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAACCCTGGCTAGCGTGTAC |
| Suggested primer 2: | AGCAGAAGGAGGAGGAGGAG |