| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | - |
| Strain: | CLIP2.064954 |
| Chromosome: | chromosome 12 |
| Location: | 6341852 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g537000 | (1 of 1) PF02041 - Auxin binding protein (Auxin_BP) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGCTTCGATTCTTGTGCATTAGGTTGTTCCGAACCACTGGACGCCGTGC |
| Internal bar code: | GAGGCCCAGTTACCGCTTAGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 540 |
| LEAP-Seq percent confirming: | 61.5385 |
| LEAP-Seq n confirming: | 16 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGCTATGAGGCTCACTACG |
| Suggested primer 2: | ATCAATGACACAGCGGTCGT |