Insertion junction: CLIP2.064963_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.064963
Chromosome:chromosome 9
Location:5197598
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre09.g801068 (1 of 5) 2.4.1.17 - Glucuronosyltransferase / Uridine diphosphoglucuronyltransferase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACTGAGGATGACGTCGAAGCGGCCGCGGGCCACCATGCGGTTTATACTG
Internal bar code:ATGGGGTGGACGTAATAGGAGG

Confirmation - LEAP-Seq

LEAP-Seq distance:1120
LEAP-Seq percent confirming:48.4848
LEAP-Seq n confirming:16
LEAP-Seq n nonconfirming:17
LEAP-Seq n unique pos:33

Suggested primers for confirmation by PCR

Suggested primer 1:CATGTGCTGGTGTGTGTGTG
Suggested primer 2:ACCCGAACCCAAACCAGATC