| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.064966 |
| Chromosome: | chromosome 1 |
| Location: | 1696642 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g009350 | AIH2 | Agmatine iminohydrolase; (1 of 2) 3.5.3.12 - Agmatine deiminase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGGGAGGTGGAAGTGGGCACGTGAGAGATGTGGGCGGTAGGGGGTGGAC |
| Internal bar code: | TGTCGGCTGATGTTTGGGGGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 226 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGGGAGAGGGAGGAGTCAG |
| Suggested primer 2: | GCGTCCTCGGTGTTCTCTAG |