| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.064970 |
| Chromosome: | chromosome 6 |
| Location: | 58755 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g249150 | MAPKK1 | Mitogen-Activated Protein Kinase Kinase 1; (1 of 1) PTHR24361//PTHR24361:SF408 - MITOGEN-ACTIVATED KINASE KINASE KINASE // SERINE/THREONINE-PROTEIN KINASE STE7 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGACCTAGGCCGGACGGCTGGGGAGGACAGCATCAACCTTTCCTCCAGA |
| Internal bar code: | TTTCGTTGACGCGTTCTAGTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3522 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 42 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 42 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGTAGCGACGTGGGCATAG |
| Suggested primer 2: | AACCCTGAATTTGCGTGTGC |