| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.065004 |
| Chromosome: | chromosome 3 |
| Location: | 2548008 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g159750 | VPS36 | Subunit of ESCRT-II complex; (1 of 1) K12190 - ESCRT-II complex subunit VPS36 (VPS36, EAP45) | 3'UTR |
| Cre03.g159800 | (1 of 1) PTHR21262//PTHR21262:SF0 - GUANOSINE-3',5'-BIS DIPHOSPHATE 3'-PYROPHOSPHOHYDROLASE // GUANOSINE-3',5'-BIS(DIPHOSPHATE) 3'-PYROPHOSPHOHYDROLASE MESH1 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGAACGCTGGGATGTTGTTGTTCGGGGGCGGCATTGCCAAGGACTACTGT |
| Internal bar code: | CACGTTCGCTTAATCGAGTGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5579 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 82 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 82 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACAAAAGGCTGGGCGGTAG |
| Suggested primer 2: | ACTGTACCGACGAGTTGCTG |