| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.065006 |
| Chromosome: | chromosome 12 |
| Location: | 8370467 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g550553 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGCGCCGCACGTCCAAACGCAGGCACATACGCACGAACATGCCGCCAAA |
| Internal bar code: | TGATCTCGGTTTTTACCGATTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4738 |
| LEAP-Seq percent confirming: | 83.9506 |
| LEAP-Seq n confirming: | 68 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 81 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGCTGTTTCTTATCAGGCG |
| Suggested primer 2: | CATCCTCCGAAGCCCTGATC |