Insertion junction: CLIP2.065024_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.065024
Chromosome:chromosome 10
Location:6398728
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g801209 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTTTTCGGGATGTCATGCTAGGTCCGTAGCTAGTGATTTGGGTCCCCGGT
Internal bar code:GAATGTGGAAAACGATCCTGTA

Confirmation - LEAP-Seq

LEAP-Seq distance:1681
LEAP-Seq percent confirming:82.3529
LEAP-Seq n confirming:14
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:17

Suggested primers for confirmation by PCR

Suggested primer 1:TTCAAAACTGCTTGCCGCTC
Suggested primer 2:GCGGCAACATGGATGATGAC