| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.065060 |
| Chromosome: | chromosome 12 |
| Location: | 8415497 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g550250 | (1 of 3) PF03638 - Tesmin/TSO1-like CXC domain, cysteine-rich domain (TCR) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGAGGAGCCGCCCGCCGCCACCGCTGCGGCCTCAGCGGCGGCACGCGACA |
| Internal bar code: | GAATGCAATTGGGCAACGTGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1591 |
| LEAP-Seq percent confirming: | 16.6667 |
| LEAP-Seq n confirming: | 4 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCAACTTGGACTCGCCCAG |
| Suggested primer 2: | ATGTGCAGCAACGACCTGTA |