| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.065063 |
| Chromosome: | chromosome 16 |
| Location: | 3241895 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g665900 | 5'UTR | ||
| Cre16.g665950 | MCM8 | (1 of 1) K10737 - DNA helicase MCM8 [EC:3.6.4.12] (MCM8); Minichromosome maintenance protein | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCAGCTTTTGCAGTGCAAGGTCCGGGGTAACGCAAATTGTTTATACAGT |
| Internal bar code: | CTCTTGTGAGACATAGTGGCCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4396 |
| LEAP-Seq percent confirming: | 92.4528 |
| LEAP-Seq n confirming: | 49 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 53 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTCCGGTTGTAGCTTGAGC |
| Suggested primer 2: | CATTTGCCCATGAGCTCAGC |