| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.065071 |
| Chromosome: | chromosome 11 |
| Location: | 3084287 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g475000 | IFT121,IFT122B,FAP118 | Intraflagellar Transport Protein 121; (1 of 1) PTHR16517:SF1 - WD REPEAT-CONTAINING PROTEIN 35 | 5'UTR |
| Cre11.g475050 | (1 of 1) K17973 - N-terminal acetyltransferase B complex non-catalytic subunit (NAA25, MDM20) | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGCGTCGCTTGCAACGCGTTCTACTGTTCTATTGCTTTCCTATCCAACA |
| Internal bar code: | TAAGATCGTGTCGGGGTGTATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4808 |
| LEAP-Seq percent confirming: | 96.5517 |
| LEAP-Seq n confirming: | 28 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 29 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCATGCTTCAGAGACCCCAG |
| Suggested primer 2: | GAGGAGAGGAGGGATGGTGT |