| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.065084 |
| Chromosome: | chromosome 4 |
| Location: | 2472249 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g221950 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGTAACTTAAGAGTTAGTGACTGGACACGATATAGCTAAAATGCATGTG |
| Internal bar code: | TATTACCATTTAAATTTGAAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1830 |
| LEAP-Seq percent confirming: | 95.2381 |
| LEAP-Seq n confirming: | 40 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 42 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACTCGCCGCATTTACGAAG |
| Suggested primer 2: | CTGTTATTTGGCAGGCGTGG |