| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.065086 |
| Chromosome: | chromosome 7 |
| Location: | 3235097 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g334150 | (1 of 1) PTHR23097//PTHR23097:SF124 - TUMOR NECROSIS FACTOR RECEPTOR SUPERFAMILY MEMBER // SUBFAMILY NOT NAMED | 3'UTR | |
| Cre07.g334200 | HEL38 | (1 of 1) K13116 - ATP-dependent RNA helicase DDX41 [EC:3.6.4.13] (DDX41, ABS); DEAD box ATP-dependent RNA helicase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTCTGGCCACCCTTGACCAAACTGCTAATAGCTTTGCGCACTTCTCGTC |
| Internal bar code: | GGGCGGGCTTTGTTATGGTTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5185 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 133 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 133 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCTGCGTTTGTTGTACGTG |
| Suggested primer 2: | GGACAGGTGGTGATCTGGTG |