Insertion junction: CLIP2.065090_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.065090
Chromosome:chromosome 14
Location:1086026
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre14.g614500 BTB/POZ domain containing protein; (1 of 25) IPR000210//IPR011333 - BTB/POZ domain // POZ domain 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AAGTCAAAATATACTTGTACGCTATTATCAGGATGCAAACACACCGCTGA
Internal bar code:GGACATTAGAGGGCCACGCGTG

Confirmation - LEAP-Seq

LEAP-Seq distance:1318
LEAP-Seq percent confirming:80.0
LEAP-Seq n confirming:8
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:10

Suggested primers for confirmation by PCR

Suggested primer 1:CCGTTGCATTACGCTAGTGC
Suggested primer 2:GTCGTCCTTGTCCTCGATCC