| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.065094 |
| Chromosome: | chromosome 17 |
| Location: | 5948459 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g742132 | 5'UTR | ||
| Cre17.g742150 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCATAACGTTGCCCCGACATCGTGCTCAAAACTGCGCCCGCTACAGGCC |
| Internal bar code: | GTGTACGTGCCCGAGCCCAGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3494 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 12 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGTCACTTGTCTCCGCCAA |
| Suggested primer 2: | GCCGGCTTGTGCAAAATACA |