| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.065118 |
| Chromosome: | chromosome 12 |
| Location: | 5788565 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g532800 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGTGTGTGTTAAAGAGCACAAGGAAAACGTTGCGCAAAGACAGTGCGTG |
| Internal bar code: | ACCTAGCGCGGTTCATTCACTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 69 |
| LEAP-Seq percent confirming: | 3.0303 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 32 |
| LEAP-Seq n unique pos: | 33 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCGCCGGTAGATGCAGAG |
| Suggested primer 2: | TTTGCTTGCAGTCACTTCGC |