Insertion junction: CLIP2.065120_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.065120
Chromosome:chromosome 3
Location:1269866
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre03.g149950 (1 of 83) IPR029044 - Nucleotide-diphospho-sugar transferases intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTTTGCAACGCCCCCCCCCCTCCTCACACACACACACACCCTGCCAGGCT
Internal bar code:AGCTCGTATTCCATAATACCGG

Confirmation - LEAP-Seq

LEAP-Seq distance:743
LEAP-Seq percent confirming:50.0
LEAP-Seq n confirming:16
LEAP-Seq n nonconfirming:16
LEAP-Seq n unique pos:32

Suggested primers for confirmation by PCR

Suggested primer 1:CTTGTGTGGCGCATCATAGC
Suggested primer 2:TTTGTCGTCGGTCGCACTAA