| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.065166 |
| Chromosome: | chromosome 6 |
| Location: | 816529 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g255350 | THI10 | (1 of 1) 2.7.1.50 - Hydroxyethylthiazole kinase; Hydroxyethylthiazole kinase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACTGGCCCCAACCGCTGCCACTGCTGCTGCCGCGGGGCCACGCGACAGC |
| Internal bar code: | CTGCTCGGGTACGCTAAGTGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1347 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 33 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 33 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAGCTTTGGCCTTTGTGTG |
| Suggested primer 2: | GCGGGCCTATGAATGGATGA |