Insertion junction: CLIP2.065167_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.065167
Chromosome:chromosome 1
Location:7911899
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g055469 (1 of 2) IPR007213//IPR011610//IPR029063 - Leucine carboxyl methyltransferase // S-adenosyl-L-methionine-dependent methyltransferase, putative // S-adenosyl-L-methionine-dependent methyltransferase 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACTCTCGCCTGCGGTGCGACCCGGTCCTGTGCTGTCGCCACACACACAGC
Internal bar code:CATCGCTGCGAGATCGTATACA

Confirmation - LEAP-Seq

LEAP-Seq distance:700
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:16
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:16

Suggested primers for confirmation by PCR

Suggested primer 1:TGGTCCCCCATGTAGAGAGG
Suggested primer 2:TAGAGGGTGAGCAGCAAAGC