Insertion junction: CLIP2.065168_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.065168
Chromosome:chromosome 3
Location:3383126
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g166500 (1 of 2) IPR000104//IPR013083 - Antifreeze protein, type I // Zinc finger, RING/FYVE/PHD-type 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTTCAGAGTTGCCAATCCTGTATCCGCCCGCAGTCTTCTAGGGTGTTGCC
Internal bar code:CCCGAGGATAAGCTCTGGGGTA

Confirmation - LEAP-Seq

LEAP-Seq distance:1899
LEAP-Seq percent confirming:98.3607
LEAP-Seq n confirming:60
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:61

Suggested primers for confirmation by PCR

Suggested primer 1:CGACATCCTACTGCCATCCC
Suggested primer 2:CAGCGGCTGAAGTAGAAGGG