Insertion junction: CLIP2.065172_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.065172
Chromosome:chromosome 6
Location:3585753
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g278128 (1 of 1) IPR000953//IPR005818//IPR016197 - Chromo/chromo shadow domain // Linker histone H1/H5, domain H15 // Chromo domain-like 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTTGGCGTAGTCCCGTAGTGTGCAGCGTACTCCCAGATCCCAGTTTGCAA
Internal bar code:CTTGGTAACTTCTAGTTGCGTG

Confirmation - LEAP-Seq

LEAP-Seq distance:1273
LEAP-Seq percent confirming:63.3803
LEAP-Seq n confirming:45
LEAP-Seq n nonconfirming:26
LEAP-Seq n unique pos:71

Suggested primers for confirmation by PCR

Suggested primer 1:GAGCACTAAAGCAATCGCGG
Suggested primer 2:CCTTTCGCTTCCCCTCACTT