Insertion junction: CLIP2.065179_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.065179
Chromosome:chromosome 1
Location:7583524
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g800139 (1 of 1) IPR026741//IPR026937//IPR027417 - Protein strawberry notch // Strawberry notch, helicase C domain // P-loop containing nucleoside triphosphate hydrolase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGACAGGTCATCGTATCGTATCGAACGCCACACAATAAAACGATTGGAGC
Internal bar code:TCTTATATAGAAAGCATACTGG

Confirmation - LEAP-Seq

LEAP-Seq distance:1704
LEAP-Seq percent confirming:94.4444
LEAP-Seq n confirming:17
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:18

Suggested primers for confirmation by PCR

Suggested primer 1:CCCTTGTCAACCTCCTGTCC
Suggested primer 2:CTCGGCACCCTCACTTCAAT