Insertion junction: CLIP2.065192_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.065192
Chromosome:chromosome 2
Location:7669099
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g147350 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGTGCCTTGACGCACACAGGGCCCTTGTGCGTGACCACTCGATCCGACGG
Internal bar code:GTCTACTTCCCTGTGCTTTGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:2494
LEAP-Seq percent confirming:98.3333
LEAP-Seq n confirming:59
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:60

Suggested primers for confirmation by PCR

Suggested primer 1:CGCTCGCAGCAATTAGGTTC
Suggested primer 2:GCGCCGGACATACTCACATA